Background free dark-field measurements via the evanescent field enables high speed cellular imaging

Recording of direct scattering in a living HELA cell culture evanescently excited by QUSCITE at 640nm
2 ms exposure time
100x NA1.47 oil, Leica Dmi8 microscope, 2×2 binning, pixel size 130 nm
Ciliary structures touch the evanescent field and scatter light
Image acquired at OICE – Optical Imaging Center Erlangen. Sample prep: Dr. Philipp Tripal.

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