Capturing Fast Dynamics with pure TIRF
The unicellular green algae Chlamydomonas, is a key model organism for studying photosynthesis and cell motility. Its flagella typically grows upto 10 to 12 µm in length with a diameter around 0.2 µm. Interestingly, the flagella exhibits coordinated beating patterns which allows it to swim at speeds of 100-200 microns per second, rendering its dynamics essential for its motility. Total Internal Reflection Fluorescence (TIRF) microscopy has been instrumental in visualizing flagellar processes in Chlamydomonas.
Description
Video showing the dynamics of Chlamydomonas with endogenously green fluorescent tagged ciliary coat protein FMG1B. Images were acquired using a Zeiss Airyscan microscope with a 63X 1.4NA oil objective. Multiple frames were acquired with 15ms exposure time.