Capturing Fast Dynamics with pure TIRF
Description
The unicellular green algae Chlamydomonas, is a key model organism for studying photosynthesis and cell motility. Its flagella typically grows upto 10 to 12 µm in length with a diameter around 0.2 µm. Interestingly, the flagella exhibits coordinated beating patterns which allows it to swim at speeds of 100-200 microns per second, rendering its dynamics essential for its motility. Total Internal Reflection Fluorescence (TIRF) microscopy has been instrumental in visualizing flagellar processes in Chlamydomonas.