Multiplexed fluorescence imaging with DNA-encoded thermal channels
Description
The lab of Peng Yin from the University of Florida used VAHEAT for rapid temperature changes, allowing for fast channel switching (less than 30 seconds) between thermal imaging channels. Thermal-plex uses simple DNA probes that are engineered to fluoresce sequentially when, and only when, activated with transient exposure to heating spikes at different temperatures (39 °C, 48 °C, 57 °C, 65 °C and 72 °C)