Temperature sensitive microscopy in life sciences
The lab of Peng Yin from the University of Florida used VAHEAT for rapid temperature changes, allowing for fast channel switching (less than 30 seconds) between thermal imaging channels. Thermal-plex uses simple DNA probes that are engineered to fluoresce sequentially when, and only when, activated with transient exposure to heating spikes at different temperatures (39 °C, 48 °C, 57 °C, 65 °C and 72 °C)
Description
In this seminar, Daniel Böning from Interherence GmbH introduced our newly developed micro-heating system VAHEAT that enables precise temperature control in the field of view when working with immersion medium based objectives.
Philipp Tripal, from the Optical Imaging Centre Erlangen, Germany, talked about important requirements for live-cell imaging and showed a glimpse of his research on vesicle transport in neurons.
Mart Krupovic, from the Institut Pasteur in Paris, France, presented the recent advances in the understanding of the morphological and structural diversity of archaeal viruses, and uncovered some of the molecular adaptations underlying the stability of archaeal viruses in extremely hot acidic environments. Finally, he highlighted the mechanisms of virus-host interactions in Archaea, especially those that could be further understood through the development of live-cell imaging approaches.